Recombinant Mouse Interleukin-2/IL-2 Protein

Specifications

Catalog number

PKSM040308-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-2, also known as T-cell growth factor, TCGF, Aldesleukin and IL2, is a secreted protein which belongs to the IL-2 family. Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2, and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy has been found to be ineffective.

Abbreviation

Interleukin-2; IL-2

Synonyms

POIL; OTTHUMP; Aldesleukin; IL 2; Interleukin 2; Involved in regulation of T cell clonal expansion; Lymphokine; OTTHUMP00000164090; POIL2; T Cell Growth Factor; T-cell growth facter; Interleukin-2; IL2; TCGF; T-cell growth factor; Il-2

UniProt

P04351

Accession Number

P04351

Expression System

E.coli

Tag

C-His

Sequence

Ala 21-Gln 169

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

503 x 106 IU/mg.6

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.2 kDa

Observed Molecular Weight

18 kDa

Species

Mouse