Recombinant Mouse OBCAM/OPCML Protein (aa 5-313, His Tag)

Specifications

Catalog number

PKSM040373

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Opioid-binding Cell Adhesion Molecule (OBCAM), also known as OPCML, is a GPI-anchored cell adhesion molecule in the plasma membrane. This neuron-specific protein, consists of three immunoglobulin (Ig) -like domains anchored to the membrane through a glycosylphosphatidylinositol (GPI) -tail. OPCML also belongs to the member of the IgLON family, a subgroup of the immunoglobulin superfamily, consisting of three members, LAMP, OBCAM, and Neurotrimin. These molecules interact homophilically and heterophilically within the family, and OBCAM acts only as heterodimers with LAMP or Neurotrimin and possibly inhibits neurite outgrowth from cerebellar granule cells. OBCAM has been presumed to play a role as a cell adhesion/recognition molecule. Furthermore, the OPCML protein defects may play an important role in the carcinogenesis of cervical or ovarian cancers, and this gene is regarded as a candidate TSG (tumor suppressor gene) .

Synonyms

2900075O15Rik;3732419F12; AI844366; B-CAM; B930023M13Rik; C230027C17; Gm181; Gplu; Lu; Obcam

UniProt

Q6DFY2

Accession Number

NP_808574.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala5-Val313

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse OPCmL-His at 10μg/mL (100μL/well) can bind human LSAMP-Fc. The EC50 of human LSAMP-Fc is 0.04-0.1 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.7 kDa

Observed Molecular Weight

44-55 kDa

Species

Mouse