Recombinant Mouse Pleiotrophin/PTN/HB-GAM Protein (Fc Tag)

Specifications

Catalog number

PKSM040396

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

Synonyms

HARP; HB-GAM; HBBM; HBBN; HBGF-8; HBNF; Heparin-binding brain mitogen; Heparin-binding growth factor 8; OSF; OSF-1; Osf-1; Osf1; Osteoblast-specific factor 1; PTN; Pleiotrophin

UniProt

P63089

Accession Number

P63089

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Gly 33-Asp 168

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.7 kDa

Observed Molecular Weight

47 kDa

Species

Mouse