Recombinant Mouse Cystatin E/CST6 Protein (His Tag)

Specifications

Catalog number

PKSM040441

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.

Synonyms

1110017E11Rik; N28197; ichq

UniProt

Q9D1B1

Accession Number

NP_082899.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 152

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate ZFR-AMC, R&D Systems, Catalog # ES009.The IC50 is < 20 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.2 kDa

Observed Molecular Weight

20 kDa

Species

Mouse