Recombinant Mouse PTPN6/SH-PTP1 Protein (aa 207-597, His & GST Tag)

Specifications

Catalog number

PKSM040450

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PTPN6 is an enzyme which belongs to the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of PTPN6 contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of PTPN6 with its substrates. PTPN6 is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. It has been shown that PTPN6 interacts with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.

Synonyms

70Z-SHP; Hcph; PTPTY-42; Ptp1C; SH-PTP1; SHP-1; hcp; me

UniProt

P29351

Accession Number

P29351-2

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Ala207-Lys597

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988998 phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 4 μmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

72.7 kDa

Observed Molecular Weight

63 kDa

Species

Mouse