Recombinant Mouse SLC3A2/CD98 Protein (His Tag)

Specifications

Catalog number

PKSM040473

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type II membrane protein which belongs to the SLC3A transporter family. MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. MDU1 is required for the function of light chain amino-acid transporters. It involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.

Synonyms

4F2 Protein;4F2HC Protein; AI314110 Protein; Cd98 Protein; Ly-10 Protein; Ly-m10 Protein; Ly10 Protein; Mdu1 Protein; Mgp-2hc Protein; Mouse; NACAE Protein

UniProt

P10852

Accession Number

NP_001154885.1

Expression System

HEK293 Cells

Tag

N-His

Sequence

Ala 139-Ala 565

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.2 kDa

Observed Molecular Weight

60-70 kDa

Species

Mouse