Recombinant Mouse 4-1BB/TNFRSF9 Protein (aa 1-211, Fc Tag)

Specifications

Catalog number

PKSM040474-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor– associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.

Synonyms

4-1BB; A930040I11Rik; AA408498; AI325004; CDw137; Cd137; ILA; Ly63; Secreted CD137 antigen; Tnfrsf9 ; Tumor necrosis factor receptor superfamily member 9

Accession Number

NP_001070976.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Leu 211

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse His-TNFSF9 at 10 μg/ml (100 μl/well) can bind mouse TNFRSF9-Fc, The EC50 of mouse TNFRSF9-Fc is 12.0-29.0 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.0 kDa

Species

Mouse