Recombinant Mouse PARP-1 Protein (His Tag)

Specifications

Catalog number

PKSM040501

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Poly (ADP-ribose) polymerase 1 (PRAP1), also known as NAD (+) ADP-ribosyltransferase 1 (ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly (ADP-ribosyl) ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly (ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Synonyms

5830444G22Rik; AI893648; ARTD1; Adprp; Adprt1; C80510; PARP; PPOL; parp-1; sPARP-1

Accession Number

NP_031441.2

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Met 1-Trp 1014

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

115 kDa

Observed Molecular Weight

75 kDa

Species

Mouse