Recombinant Mouse Syndecan-4/SDC4 Protein (Fc Tag)

Specifications

Catalog number

PKSM040519

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with a number of signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.

Synonyms

AA959608; AW108331; Ryudocan core protein; SDC4; SYND4; Synd4; ryudocan; syndecan-4

UniProt

O35988

Accession Number

O35988

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met1-Val146

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human MDK at 10 μg/ml (100 μl/well) can bind mouse SDC4-Fc with a linear range of 0.16-1. 25 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.5 kDa

Observed Molecular Weight

33 kDa

Species

Mouse