Recombinant Mouse Semaphorin-4D/SEMA4D Protein (His Tag)

Specifications

Catalog number

PKSM040568

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Semaphorin 4D (SEMA4D or CD1) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D) . Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

Synonyms

CD100; M-Sema G; SEMA4D; Sema J; Semacl2; Semaj; Semaphorin-4D; Semaphorin-C-like 2; Semaphorin-J; coll-4

UniProt

O09126

Accession Number

NP_038688.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Arg 733

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 96 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind human SEMA4A in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

80.2 kDa

Observed Molecular Weight

100-110 kDa

Species

Mouse