Recombinant Mouse c-MET/HGFR Protein (Fc Tag) (Fc Tag)

Specifications

Catalog number

PKSM040581-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Synonyms

AI838057; HGF; HGFR; Par4; c-Met

Accession Number

NP_032617.2

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Asn 929

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human HGF at 2 μg/ml (100 μl/well) can bind mouse HGFR with a linear ranger of 1. 28-32ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32&96 kDa

Observed Molecular Weight

43&115-120 kDa

Species

Mouse