Recombinant Mouse EphB4/HTK Protein (Fc Tag)

Specifications

Catalog number

PKSM040599

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Synonyms

AI042935; Htk; MDK2; Myk1; Tyro11

UniProt

P54761

Accession Number

P54761-1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Ala 539

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse EPHB2-His at 10 μg/mL (100 μl/well) can bind mouse EPHB4-Fc, The EC50 of mouse EPHB4-Fc is 15 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

84.7 kDa

Observed Molecular Weight

110 kDa

Species

Mouse