Recombinant Mouse FOLR1 Protein (aa 1-231, His Tag)

Specifications

Catalog number

PKSM040605

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by FOLR1 gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. Folate receptor α (FRα) is the most important subunit of Folate receptor and the alpha isoform has been shown to be selectively overexpressed in cancer types like breast and ovarian cancer compared to normal breast and ovarian epithelial cells. It was determined that Folate receptor α exhibits a limited expression on the apical surfaces of the epithelial cells of normal lung, breast, thyroid, parathyroid, and kidney tissues. For their uptake of folate, normal cells rely almost exclusively on the reduced folate carrier, whereas many carcinomas and myeloid leukemia cells overexpress a high-affinity FR on their surfaces, perhaps reflecting their increased need for folate to support rapid cell division

Synonyms

Adult folate-binding protein; FBP; FBP1; FOLR; FOLR1; FR-alpha; Folate receptor; Folate receptor 1; Folbp-1; Folbp1; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18; adult; folate binding protein; folate receptor 1 (adult) ; folate receptor alpha

UniProt

P35846

Accession Number

NP_032060.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Met 231

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.6 kDa

Observed Molecular Weight

33-37 kDa

Species

Mouse