Recombinant Mouse SHP2/PTPN11 Protein (His Tag)

Specifications

Catalog number

PKSM040671

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase (PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.

Synonyms

2700084A17Rik; AW536184; PTP1D; PTP2C; SAP-2; SH-PTP2; SH-PTP3; SHP-2; Shp2; Syp

UniProt

P35235

Accession Number

P35235-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Arg593

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006) . The specific activity is > 1 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

69.5 kDa

Observed Molecular Weight

65 kDa

Species

Mouse