Recombinant Mouse PRLR/Prolactin Receptor Protein (His & Fc Tag)

Specifications

Catalog number

PKSM040675

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â … cytokine receptor superfamily, and contains two fibronectin type-â … ¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR) . Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

Synonyms

PRL-R; PRLR; Pr-1; Pr-3; Prlr; Prlr-rs1; Prolactin R; Prolactin receptor

UniProt

Q08501

Accession Number

NP_035299.4

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Asp 229

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.5 kDa

Observed Molecular Weight

65-70 kDa

Species

Mouse