Recombinant Mouse Trypsin 2/PRSS2 Protein (His Tag)

Specifications

Catalog number

PKSM040711

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein which belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247) . PRSS2 contains onepeptidase S1 domain. It is secreted into theduodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactivezymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated bycholecystokinin through the common activation mechanism.

Synonyms

Prss2; TRY8; TRYP; Ta; Tesp4; Try2

UniProt

P07146

Accession Number

NP_033456.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 246

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog #27096) . The specific activity is > 1500 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.2 kDa

Observed Molecular Weight

32 kDa

Species

Mouse