Recombinant Mouse CD16 Protein (His Tag)

Specifications

Catalog number

PKSM040733-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (Fcγ R) . Fcγ R is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16) . CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/Fcγ RIIIa and CD16b/Fcγ RIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Synonyms

CD16 Protein; Mouse

UniProt

P08508

Accession Number

P08508-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr 215

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse FCGR3-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.12-0.28 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.6 kDa

Species

Mouse