Recombinant Mouse PPM1A Protein (His Tag)

Specifications

Catalog number

PKSM040761

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ -activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ -induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

Synonyms

2310003C21Rik;2900017D14Rik; AI427932; AU017636; MMPa-2; MPPa-1

UniProt

P49443

Accession Number

NP_032936.1

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Trp 382

Endotoxin

Please contact us for more information.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.3 kDa

Observed Molecular Weight

43.3 kDa

Species

Mouse