Recombinant Mouse CNDP2/CPGL/PEPA Protein (His Tag)

Specifications

Catalog number

PKSM040782

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytosolic non-specific dipeptidase, also known as CNDP dipeptidase 2, Glutamate carboxypeptidase-like protein 1, Peptidase A, CNDP2 and CN2, is a cytoplasm protein which belongs to thepeptidase M20A family. CNDP2 / CPGL is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 / CPGL hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Itmay be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Isoform1 of CNDP2 / CPGL is ubiquitously expressed with higher levels in kidney and liver (at protein level) . Isoform2 of CNDP2 / CPGL is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 / CPGL is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis.

Synonyms

CNDP dipeptidase 2; CNDP2; Cytosolic non-specific dipeptidase; Dip-2; Glutamate carboxypeptidase-like protein 1; Pep-1; Pep1

UniProt

Q9D1A2

Accession Number

NP_075638.2

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Asn 475

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave carnosine (β-Ala-His) in a two step assay (mouse CNDP2 concentration 10 μg/ml) . The specific activity is > 40 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

54.0 kDa

Species

Mouse