Recombinant Mouse KIRREL1/NEPH1 Protein (His Tag)

Specifications

Catalog number

PKSM040812

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

Synonyms

Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Kirrel1; Neph1; Nephrin-like protein 1

UniProt

Q80W68

Accession Number

NP_570937.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Leu 525

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells (ATCC: CRL2279) . When cells are added to coated plates (30 μg/mL, 100 μL/well), > 40% cells will adhere specifically after 90 minutes at 37 °C.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.8 kDa

Observed Molecular Weight

65-70 kDa

Species

Mouse