Recombinant Mouse IL6ST/CD130 Protein (His Tag)

Specifications

Catalog number

PKSM040841-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM) . CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.

Abbreviation

IL6ST/CD130

Synonyms

5133400A03Rik; AA389424; BB405851; CD130; D13Ertd699e; gp130

UniProt

Q00560

Accession Number

NP_034690.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu 617

Applications

Cell culture; ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind mouse IL11Ra in a functional ELISA. 2. Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 10 ng/mL recombinant human IL6 sR and 20 ng/mL recombinant human IL6.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

68.0 kDa

Observed Molecular Weight

80-90 kDa

Species

Mouse