Recombinant Mouse APCS/SAP Protein (His Tag)

Specifications

Catalog number

PKSM040858

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serum amyloid P component (SAP) is the identical serum form of amyloid P component (AP), a highly preserved plasma protein named for its ubiquitous presence in amyloid deposits. As a normal plasma protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". Serum amyloid P component represents another member of the pentraxin family, a highly conserved group of molecules that may play a role in innate immunity. SAP is a key negative regulator for innate immune responses to DNA and may be partly responsible for the insufficient immune responses after DNA vaccinations in humans. SAP suppression may be a novel strategy for improving efficacy of human DNA vaccines and requires further clinical investigations.

Synonyms

9.5S alpha-1-glycoprotein; APCS; MGC88159; PTX2; PTX2serum amyloid P-component; SAP; SAP pentaxin-related; Serum amyloid P

UniProt

P12246

Accession Number

NP_035448.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu 224

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized mouse APCS at 10 μg/ml (100 μl/well) can bind biotinylated human Fibronectin Fragment 2 with a linear ranger of 0.625-5 μg/ml. 2. Measured by its ability to bind mouse CD64-AVI in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.3 kDa

Observed Molecular Weight

28 kDa

Species

Mouse