Recombinant Mouse Nogo Receptor/NgR Protein (His & Fc Tag)

Specifications

Catalog number

PKSM040862

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI) -anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS) . As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD) . Additionally, RTN4R may play a role in regulating the function of the gap junctions.

Synonyms

NOGOR; NgR; NgR1; Rtn4r

UniProt

Q99PI8

Accession Number

NP_075358.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Ser 447

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized recombinant Mouse RTN4R Fc Chimera at 2 μg/ml (100 μl/well) can bind biotinylated human RTN4 (GST Tag) with a linear range of 0.16-2. 5 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

73.8 kDa

Observed Molecular Weight

95-100 kDa

Species

Mouse