Recombinant Mouse CD64/FCGR1 Protein (His&AVI Tag), Biotinylated

Specifications

Catalog number

PKSM040877-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Synonyms

AI323638; AV092959; CD64; FcgammaRI; IGGHAFC

UniProt

P26151

Accession Number

NP_034316.1

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-His-Avi

Sequence

Met 1-Pro 297

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Biotinylated Mouse CD64 recombinant protein, the EC50 is 20-50 ng/mL. 2. Labeling ratio of biotin to protein is 0.7-1 by the HABA assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.3 kDa

Observed Molecular Weight

50-55 kDa

Species

Mouse