Recombinant Mouse Latexin/LXN Protein (His Tag)

Specifications

Catalog number

PKSM040884

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to theprotease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.

Synonyms

MGC144352; MGC144353

UniProt

P70202

Accession Number

NP_058033.2

Expression System

E.coli

Tag

N-His

Sequence

Glu 2-Glu 222

Applications

Enzyme

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit carboxypeptidase-A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5'Dithiobis (2-nitrobenzoic acid) (DTNB) . The IC50 value is < 2.0 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.3 kDa

Observed Molecular Weight

32 kDa

Species

Mouse