Recombinant Mouse L-Selectin/SELL Protein (His & Fc Tag)

Specifications

Catalog number

PKSM040886

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naï ve and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.

Synonyms

CD62 antigen-like family member L; CD62L; L-selectin; LECAM-1; LECAM1; Leukocyte adhesion molecule 1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1; Lymph node homing receptor; Lymphocyte antigen 22; Sell

UniProt

P18337

Accession Number

NP_035476.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Asn 332

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to SELL coated plates (10 μg/mL, 100 μL/well) approximately > 60% cells will adhere specifically.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

61.0 kDa

Observed Molecular Weight

100-110 kDa

Species

Mouse