Recombinant Mouse CD32/FCGR2B Protein (His Tag)

Specifications

Catalog number

PKSM040902-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16) . CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

Synonyms

AI528646; CD32; F630109E10Rik; Fc[g]RII; FcgRII; Fcgr2; Fcgr2a; Fcr-2; Fcr-3; Ly-17; Ly-m20; LyM-1; fcRII

Accession Number

NP_001070657.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Arg 217

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse FCGR2B-His (CD32) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.13-0.29 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.7 kDa

Observed Molecular Weight

35-40 kDa

Species

Mouse