Recombinant Mouse HVEM/TNFRSF14 Protein (His & Fc Tag)

Specifications

Catalog number

PKSM040930

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β ) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA) . HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.

Synonyms

ATAR; Atar; HVEM; Herpesvirus entry mediator; HveA; TNF receptor-like molecule; TR2; Tnfrsf14; Tumor necrosis factor receptor superfamily member 14; another TRAF-associated receptor

UniProt

Q80WM9

Accession Number

NP_849262.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Gln 206

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse HVEM-Fch at 10 μg/mL (100 μl/well) can bind biotinylated mouse BTLA-Fc, The EC50 of biotinylated mouse BTLA-Fcis 64-96 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.4 kDa

Observed Molecular Weight

65 kDa

Species

Mouse