Recombinant Mouse BMPRIA/ALK-3 Protein (His & Fc Tag)

Specifications

Catalog number

PKSM040937

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGFB) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF) -beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.

Synonyms

ALK-3; Activin receptor-like kinase 3; Acvrlk3; BMP type-1A receptor; BMP-2/BMP-4 receptor; BMPR-1A; BMPR-IA; Bmpr; Bone morphogenetic protein receptor type-1A; CD292; SKR5; Serine/threonine-protein kinase receptor R5

UniProt

P36895

Accession Number

NP_033888.2

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Arg 152

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.1-0.3 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

42 kDa

Observed Molecular Weight

60 kDa

Species

Mouse