Recombinant Mouse Carbonic Anhydrase 14/Car14 Protein (His Tag)

Specifications

Catalog number

PKSM040972-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Ca14, also known as Carbonic anhydrase 14, is a member of large family of zinc metalloenzymes .It could catalyze reversible hydration of carbon dioxide. The reaction is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Fifteen CA isoforms have been reported so far. They have different patterns of tissue-specific expression and physiologic roles. Some CAs may serve as markers for tumors and hypoxia. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C- terminal segment with several potential phosphorylation sites. A subset of CAs lack CA activity due to point mutations but retain esterase function. CA14 is widely expressed in the central nervous system

Abbreviation

Carbonic Anhydrase 14; Car14

Synonyms

CA-XIV; CA14; Carbonate Dehydratase XIV; Carbonic Anhydrase 14; Carbonic Anhydrase XIV

UniProt

Q9WVT6

Accession Number

Q9WVT6

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala16-Met290

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

31.8 kDa

Observed Molecular Weight

41-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Species

Mouse