Recombinant Mouse CCL2/MCP-1 Protein (His Tag)

Specifications

Catalog number

PKSM040978-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

C-C motif chemokine 2 (CCL2) is a member of the C-C or β chemokine family. Mouse CCL2 shares 82% amino acid (aa) identity with rat CCL2 over the entire sequence, and 58%, 56%, 55%, 53% and 53% aa identity with human, equine, porcine, bovine and canine CCL2, respectively. Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source. In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2. Additionally, it promotes Th2 polarization in CD4+ T cells. CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.

Abbreviation

CCL2; MCP-1

Synonyms

C-C motif chemokine 2; Ccl2; Je; MCP-1; Mcp1; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; Scya2 ; Small-inducible cytokine A2

UniProt

P10148

Accession Number

P10148

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln24-Asn148

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

14.7 kDa

Observed Molecular Weight

20-36 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Mouse