Recombinant Mouse CX3CL1/Fractalkine Protein (His Tag)

Specifications

Catalog number

PKSM040991-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fractalkine (CX3CL1) is a single-pass type I membrane protein and belongs to the intercrine delta family. It consists of an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. CX3CL1 exists in two forms: as a membrane-anchored or as a shed 80-95K glycoprotein. Soluble CX3CL1 is generated by limited proteolysis on the cell surface, and a disintegrin and metallopeptidase 10 (ADAM10) and ADAM17/tumor necrosis factor-α-converting enzyme (ADAM17/TACE) participate in this shedding. It has been suggested that ADAM10 acts in the constitutive shedding, and ADAM17 acts in response to cell activation. The protein may play a role in regulating leukocyte adhesion and migration processes at the endothelium.

Abbreviation

CX3CL1; Fractalkine

Synonyms

ABCD-3; C-X3-C motif chemokine 1; C3Xkine; CX3C membrane-anchored chemokine; CXC3; CXC3C; Cx3c; Fkn; Fractalkine; NTN; NTT; Neurotactin; SCYD1; Scyd1; Small-inducible cytokine D1

UniProt

O35188

Accession Number

O35188

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln25-Arg337

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.3 kDa

Observed Molecular Weight

50-65 kDa

Species

Mouse