Recombinant Mouse Exostosin-Like 2/EXTL2 Protein (His Tag)

Specifications

Catalog number

PKSM041014-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Exostosin-like 2 (EXTL2) is a member of the exostosin (EXT) -related family which contains five members: EXT1, EXT2, EXTL1, EXTL2, and EXTL3. Studies have shown that EXT gene family members have the activities of heparan sulfate-synthesizing glycosyltransferases. EXT1 and EXT2, which have been identified as causal genes for hereditary multiple exostoses, have HS-GlcAT-II and GlcNAcT-II activities. EXTL1 has GlcNAcT-II activity and EXTL3 has GlcNAcT-I and -II activities. EXTL2 has GlcNAcT-I and N-acetylgalactosaminyltransferase activities, and transfers a GlcNAc residue to the tetrasaccharide linkage region when this region is phosphorylated by a xylose kinase 1 (FAM20B) and thereby terminate chain elongation. In mice, lack of EXTL2 causes glycosaminoglycan (GAG) overproduction and structural changes of GAGs associated with pathological processes.

Abbreviation

Exostosin-Like 2; EXTL2

Synonyms

4-N-acetylhexosaminyltransferase EXTL2; Alpha-1; Alpha-GalNAcT EXTL2; EXT-related protein 2; Exostosin-like 2; Extl2; Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase

UniProt

Q9ES89

Accession Number

Q9ES89

Expression System

HEK293 Cells

Tag

N-His

Sequence

Asn43-Met330

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.6 kDa

Observed Molecular Weight

35 kDa

Species

Mouse