Recombinant Mouse CD64/FCGR1 Protein (His Tag)

Specifications

Catalog number

PKSM041015-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD64, also known as Fc-gamma receptor 1 (FcγRI), is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity. After binding IgG, CD64 interacts with an accessory chain known as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. CD64 is composed of a signal peptide, three extracellular immunoglobulin domains of the C2-type used to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 mediates endocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide production. It is normally expressed on the surfaces of monocytes and macrophages.

Abbreviation

CD64; FCGR1

Synonyms

CD64; Fc-gamma RI; FcRI; FcgammaRI; High affinity immunoglobulin gamma Fc receptor I; IGGHAFC; IgG Fc receptor I

UniProt

P26151

Accession Number

P26151

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu25-Pro297

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

31.5 kDa

Observed Molecular Weight

40-60 kDa

Species

Mouse