Recombinant Mouse GM-CSF/CSF2 Protein (His Tag)

Specifications

Catalog number

PKSM041032-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.

Abbreviation

GM-CSF; CSF2

Synonyms

CSF; CSF2; Colony-Stimulating Factor; GM-CSF; GMCSF; Granulocyte-Macrophage Colony-Stimulating Factor; Molgramostin; Sargramostim

UniProt

P01587

Accession Number

P01587

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala18-Lys141

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for this effect is 40-170 pg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.1 kDa

Observed Molecular Weight

18-30 kDa

Species

Mouse