Recombinant Mouse IL-1RA/IL1RN Protein

Specifications

Catalog number

PKSM041046-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1 beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouse Il1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26% and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.

Abbreviation

IL-1RA; IL1RN

Synonyms

IL1F; MVCD; IL-1ra; F630041P17Rik; DIRA; ICIL-1RA; IL1 inhibitor; IL1F3; IL1RA; IL-1ra3; IL-1RN; Interleukin-1 Receptor Antagonist Protein; IRAP; MVCD4; Il1rn; F630041P17Rik

UniProt

P25085

Accession Number

P25085

Expression System

E.coli

Tag

C-His

Sequence

Arg27-Gln178

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.3 kDa

Observed Molecular Weight

17 kDa

Species

Mouse