Recombinant Mouse IL12RB2/IL12R-beta 2 Protein (Fc Tag)

Specifications

Catalog number

PKSM041052-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The IL12 receptor complex, formed by IL12RB1 and IL12RB2, mediates the type I immune responses of various types of lymphocytes. Its ligand, IL12, is a heterodimeric cytokine composed of IL-12p35 and IL-12p40 subunits that are linked via disulfide bonds. Ligation of IL-12 to its receptor involves the binding of IL-12p35 to IL12RB1 and IL-12p40 to IL12RB2. This will result in the activation of tyrosine kinase 2 (TYK2), which is associated with the IL12RB1 chain and Janus kinase 2 (JAK2), which is associated with the IL12RB2 chain. Activated TYK2 and JAK2 direct the phosphorylation of STAT4. IL12RB1 is present on all lymphocytes, while the expression of IL12RB2 is tightly regulated. It has shown that the expression of IL12RB2 is limited to Th2 cells. IL12RB2 subunit plays an important role in Th1 cell differentiation, since its absence leads to an abortive Th1 differentiation that has dysfunctional production of Th1 effector molecules.

Abbreviation

IL12RB2; IL12R-beta 2

Synonyms

IL-12R-beta; IL-12 receptor beta; IL-12R subunit beta; IL-12 receptor subunit beta; interleukin-12 receptor subunit beta; Il12rb; IL-12RB; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12RB2; IL-12R-beta-2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2; Il12rb2

UniProt

P97378

Accession Number

P97378

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Met1-Asn637

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

98.4 kDa

Observed Molecular Weight

125-160 kDa

Species

Mouse