Recombinant Mouse IL15RA/CD215 Protein (Fc Tag)

Specifications

Catalog number

PKSM041053-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse interleukin-15 receptor subunit alpha, also known as Il15ra, is a high-affinity receptor for interleukin-15. Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits (Common gamma chain, or gamma c) to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide variety of Tand B cells and non-lymphoid cells. Human Il15ra shares 45% amino acid sequence homology with the mouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified, resulting from alternative splicing events involving different exons.

Abbreviation

IL15RA; CD215

Synonyms

Interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; Il15ra; IL-15R&alpha

UniProt

Q60819

Accession Number

Q60819

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gly33-Lys205

Applications

Cell culture

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.5 kDa

Observed Molecular Weight

60-90 kDa

Species

Mouse