Recombinant Mouse IL-17A Protein (His Tag)

Specifications

Catalog number

PKSM041054-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively. As IL-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.

Abbreviation

IL-17A

Synonyms

IL-17CTLA; Cytotoxic T-lymphocyte-associated antigen; IL-17Acytotoxic T-lymphocyte-associated protein; CTLA8cytotoxic T-lymphocyte-associated serine esterase; CTLA 8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Ctla9; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; Interleukin-17A; IL-17; CTLA-8; Il17a; Cytotoxic T-lymphocyte-associated antigen 8; Ctla8; Il17; CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8

UniProt

Q62386

Accession Number

Q62386

Expression System

HEK293 Cells

Tag

C-His

Sequence

Thr22-Ala158

Applications

Cell culture

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1. 25 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

16.2 kDa

Observed Molecular Weight

17-26 kDa

Species

Mouse