Recombinant Mouse Interleukin-12/IL-12 Protein

Specifications

Catalog number

PKSM041070-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on murine cells. It is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

Abbreviation

Interleukin-12; IL-12

Synonyms

CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta

UniProt

P43432; P43431

Accession Number

P43432&P43431

Expression System

HEK293 Cells

Tag

None

Sequence

Met23-Ser335&Arg23-Ala215

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.8&21.7 kDa

Observed Molecular Weight

40-55&20-28 kDa

Species

Mouse