Recombinant Mouse Interleukin-18/IL-18 Protein (His Tag)

Specifications

Catalog number

PKSM041079-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-18 (IL-18) is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.

Abbreviation

Interleukin-18; IL-18

Synonyms

Interleukin; IL-1F; Ig; Il-Igif; Il-18; IFN-gamma-inducing factor; Il; IL-1 gamma; IL-1F4; IL-1g; Interferon gamma-inducing factor; Interferon-gamma-inducing factor; Interleukin-1 gamma; Interleukin-18; Igif; Il18; Igif; Il-; Il-18

UniProt

P70380

Accession Number

P70380

Expression System

E.coli

Tag

C-His

Sequence

Asn36-Ser192

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.1 kDa

Observed Molecular Weight

18 kDa

Species

Mouse