Recombinant Mouse Interleukin-33/IL-33 Protein

Specifications

Catalog number

PKSM041090-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.

Abbreviation

Interleukin-33; IL-33

Synonyms

DVS; NF-HEV and IL- 1F; Interleukin-1 family member; C9orf; C9orf26; DVS27; Interleukin-1 family member 11; NF-HEV and IL- 1F11; NKHEV; IL33; Interleukin-33; IL-33; Interleukin 33; IL1F11; NFEHEV; NF-HEV

UniProt

Q8BVZ5

Accession Number

Q8BVZ5

Expression System

E.coli

Tag

C-His

Sequence

Ser109-Ile266

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50 2 x 107 IU/mg.7

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.5 kDa

Observed Molecular Weight

17-25 kDa

Species

Mouse