Recombinant Mouse IL-5RA/IL-5 Rα Protein (Human Cells, His Tag)

Specifications

Catalog number

PKSM041096-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 5 Receptor alpha (IL-5 Rα), also known as CD125, is a hematopoietin receptor that plays a dominant role in eosinophil biology. Mature mouse IL-5 Rα consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 Rα and the transmembrane common β chain (βc/CD131) which is shared with the receptor complexes for IL-3 and GM-CSF. IL-5 Rα binds IL-5 at low affinity and then associates with preformed βc oligomers to form the signaling-competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5 Rα followed by eosinophilic differentiation and activation. IL-5 Rα also promotes the differentiation of basophils and B cells. Exposure of mature eosinophils to IL-5 attenuates their IL-5 responsiveness by inducing the down-regulation of surface IL-5 Rα and increased production of soluble IL-5 Rα.

Abbreviation

IL-5RA; IL-5 Rα

Synonyms

CD125; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5RA; IL-5R-alpha; Interleukin-5 receptor subunit alpha; Il5r; Il5ra; CDw125; IL-5Rα Il5r

UniProt

P21183

Accession Number

P21183

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp18-His339

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.6 kDa

Observed Molecular Weight

48 kDa

Species

Mouse