Recombinant Mouse RANK/TNFRSF11A Protein (His Tag)

Specifications

Catalog number

PKSM041130-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptor activator of NF-κB (RANK, TNFRSF11A) belongs to one member of tumor necrosis factor receptor family.It is a receptor for TNFSF11/RANKL/TRANCE/OPGL. This gene encodes a type 1 membrane protein with a 30 amino acids (aa) signal peptide, 184 aa extracellular region , a 20 aa transmembrane domain and a 391 aa cytoplasmic region. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is ubiquitous highly expressed in trabecular bone, thymus, small intestine, lung, brain and kidney, but weakly expressed in spleen and bone marrow. After binding its ligand RANKL, RANK can activate signaling pathways such as NF-κB, JNK, ERK, p38, and Akt/PKB, through TRAF protein phosphorylation. RANK/TNFRSF11A signaling is largely considered to be growth promoting and apoptosis reducing such as the effects observed in osteoclasts. RANK/TNFRSF11A was also found to be involved in the regulation of interactions between T-cells and dendritic cells.

Abbreviation

RANK; TNFRSF11A

Synonyms

CD265; CD265 antigen; NFKB activator; ODFR; Osteoclast differentiation factor receptor; Receptor activator of NF-KB; TNFRSF11A; TRANCE R; TRANCE receptor; TRANCER; tumor necrosis factor receptor superfamily member 11A

UniProt

O35305

Accession Number

O35305

Expression System

HEK293 Cells

Tag

C-His

Sequence

Val31-Ser214

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.3 kDa

Observed Molecular Weight

26-30 kDa

Species

Mouse