Recombinant Mouse Semaphorin-4A/SEMA4A Protein (His Tag)

Specifications

Catalog number

PKSM041139-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Semaphorin-4A (SEMA4A) belongs to the semaphorin family which contains a Ig-like C2-type domain, a PSI domain and a Sema domain. SEMA4A is expressed from day 10 in the embryo, and low levels are found between days 10-12. SEMA4A is a cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. It plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A promotes phosphorylation of TIMD2, inhibits angiogenesis, and promotes axon growth cone collapse, Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Abbreviation

Semaphorin-4A; SEMA4A

Synonyms

SemB; Sema B; Sema4a; Semab; Semaphorin-4A; Semaphorin-B

UniProt

Q62178

Accession Number

Q62178

Expression System

HEK293 Cells

Tag

C-His

Sequence

Thr33-His682

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

72.7 kDa

Observed Molecular Weight

70-90 kDa

Species

Mouse