Recombinant Mouse SIRPA/CD172a Protein (MIgG2a Tag)

Specifications

Catalog number

PKSM041146-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SIRPα is a type I transmembrane glycoprotein.It contains two Ig-like C1-type domains and one Ig-like V-type domain. Mouse SIRP alpha ECD shares 61%, 75%, 62%, 61%, and 59% aa sequence identity with human, rat, equine, bovine, and porcine SIRP alpha, respectively.SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

Abbreviation

SIRPA; CD172a

Synonyms

CD172 Antigen-Like Family Member A; CD172a; MFR; SHPS1; SIRP; SIRPA; Signal-Regulatory Protein Alpha-1; Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1

UniProt

Q6P6I8

Accession Number

Q6P6I8

Expression System

HEK293 Cells

Tag

C-mIgG2a Fc

Sequence

Lys32-Asn372

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

65.1 kDa

Observed Molecular Weight

90-120 kDa

Species

Mouse