Recombinant Mouse TREM2 Protein (Fc Tag)

Specifications

Catalog number

PKSM041175-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Triggering receptor expressed on myeloid cells-2 (TREM-2) is a cell surface receptor primarily expressed on macrophages, osteoclasts, microglia and dendritic cells. TREM-2 is one member of the TREM family, inhibiting the releasing of inflammatory mediators, so it is an important in vivo anti-inflammatory receptor. TREM-2 consists of an 18 aa signal sequence, a 153 aa extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. A soluble form of TREM-2 (TREM-2b) created by alternate splicing diverges at aa 161. TREM-2 transduces intracellular signals through the adaptor DAP12. After binding of TREM-2 with ligand, the TREM-2/DAP12 (dead-cell-activated-receptor-associated protein) -mediated signal transduction pathway causes a series of intracellular protein tyrosine phosphorylation reactions and enzymatic reactions, which then activate the myeloid cells and participate T cell responses.

Abbreviation

TREM2

Synonyms

TREM-2; TREM-2b; Trem2; Trem2a; Trem2b; Trem2c; Triggering Receptor Expressed on Myeloid Cells 2b; Triggering receptor expressed on monocytes 2; Triggering receptor expressed on myeloid cells 2

UniProt

Q99NH8

Accession Number

Q99NH8

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Leu19-Pro168

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.7 kDa

Observed Molecular Weight

55-70 kDa

Species

Mouse