Recombinant Mouse VEGF-A/VEGF164 Protein

Specifications

Catalog number

PKSM041180-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Vascular endothelial growth factor (VEGF or VEGF-A), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF/VEGF growth factor family that is characterized by a cystine knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 120, 164 and 188 aa found in mouse. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-/KDR) on endothelial cells.Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells.It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Abbreviation

VEGF-A; VEGF164

Synonyms

VEGF-A; VEGF164; VEGFA; VEGFA164; VPF; Vascular endothelial growth factor A; Vascular permeability factor

UniProt

Q00731

Accession Number

Q00731-2

Expression System

P.pastoris

Tag

None

Sequence

Ala27-Arg190

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.3 kDa

Observed Molecular Weight

18-22 kDa

Citation 01

https://www.mdpi.com/2072-6694/13/9/2066

Species

Mouse