Recombinant Mouse ESM1/Endocan Protein (Human Cells, His Tag)

Specifications

Catalog number

PKSM041193-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Endothelial cell-specific molecule–1 (ESM-1) —so-called endocan—is a novel endothelium derived soluble dermatan sulfate proteoglycan (PG) that is constitutively expressed by endothelial cells in lungs and kidneys and can be detected in human blood. It is encoded by the ESM1 gene. The expression of this gene is regulated by several cytokines and growth factors, such as vascular endothelial growth factor. Inflammatory cytokines, such as interleukin (IL) -1β and tumor necrosis factor (TNF) -α, stimulate the upregulation of endocan mRNA and the secretion of endocan from endothelial cells. The binding of circulating endocan to leukocyte ligand for ICAM-1—Lymphocyte Function-associated Antigen-1 (LFA-1) and to leukocyte ligand for VCAM-1—Very Late Antigen-4 (VLA-4) is important in leukocyte adhesion and interaction with activated endothelium. Endocan is a key player in the regulation of major processes such as cell adhesion in inflammatory disorders and tumor progression.

Abbreviation

ESM1; Endocan

Synonyms

ESM-1; Endocan; Endothelial cell-specific molecule 1; Esm1

UniProt

Q9QYY7

Accession Number

Q9QYY7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Trp20-Arg184

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.0 kDa

Observed Molecular Weight

20 kDa

Species

Mouse