Recombinant Mouse SIGLEC3/CD33 Protein (His Tag)

Specifications

Catalog number

PKSM041206-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse myeloid cell surface antigen CD33 (CD33) is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD33 contains one Ig-like C2-type domain and one Ig-like V-type domain. CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 induces apoptosis in acute myeloid leukemia. CD33 is becoming increasingly important as a target of antibody-mediated therapy in acute myeloid leukaemia (AML) .

Abbreviation

SIGLEC3; CD33

Synonyms

CD33; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; Siglec3; gp67

UniProt

Q63994

Accession Number

Q63994

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp18-Glu240 (Gly236Arg)

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.7 kDa

Observed Molecular Weight

30-45 kDa

Species

Mouse